PCR分注済マスターミックス

製品特長

  • 操作時間を短縮
  • 反応液の調製ミスを軽減
  • カスタム注文

製品概要

Thermo Scientific ABgene 製品の耐熱性DNA Polymerase、バッファー、PCR チューブやプレートからご希望の組み合わせを選んでいただくセミカスタム製品です。チューブやプレートにPCR に必要な試薬があらかじめ分注された状態で製品を提供します。

保存温度
製品は-20℃で保存してください。4℃では最大4週間まで安定です。凍結融解を繰り返さないでください。

選択ガイド
ステップ1 でDNA Polymerase を選択した後、ステップ2 以降でバッファーやチューブ・PCR プレートを選択してい きます。 選択した酵素によって、選択するステップが異なります。

Step 1 Step 2 Step 3 Step 4 Step 5 Step 6 Step 7
Enzyme type EX Buffer Type Consumable Type Conc. & reaction MgCl2 conc. ReddyMix Pack size
AM   choice choice choice choice choice
EX choice choice choice   choice choice
TS   choice choice choice   choice

下記選択表のPDF版はこちら（50KB）からダウンロードしてください。

Step 1
Enzyme type		 AM: ThermoPrime (standard PCR)
EX: Extensor (hi-fi/long PCR)
TS: Thermo-Start (hot-start PCR)
Step 2
Buffer type
(EX ONLY)		 1: For templates up to 12kb
2: For templates > 12kb
Step 3
Consumable type		 620: Individual 0.2ml Flat Cap Thermo-Tubes
489: Individual 0.5ml Domed Cap Thermo-Tubes
337: Individual 0.2ml Domed Cap Thermo-Tubes
350: Individual 0.5ml Flat Cap Thermo-Tubes
266: Thermo-Strips with Domed Caps (8-tube strip)
1182: Thermo-Strips with Flat Caps (8-tube strip)
1113: Thermo-Strips with Domed Caps (12-tube strip)
1114: Thermo-Strips with Flat Caps (12-tube strip)
772: Low Profile Thermo-Strips with Domed Caps (8-tube strip)
773: Low Profile Thermo-Strips with Flat Caps (8-tube strip)
848: Low Profile Thermo-Strips with Domed Caps (12-tube strip)
849: Low Profile Thermo-Strips with Flat Caps (12-tube strip)
600: ABgene 96 PCR Plate Non-Skirted, Capped
700: ABgene 96 PCR Plate Non-Skirted, Low Profile, Capped
800: ABgene 96 PCR Plate Skirted, Low Profile, Capped
900: ABgene 96 PCR Plate Semi-Skirted, Capped
990: ABgene 96 PCR Plate Rigid Semi-Skirted, Capped
1000: ABgene 96 PCR Plate Rigid Skirted, Low Profile, Capped
1300: ABgene 96 Robotic PCR Plate, Capped
1100: ABgene 96 PCR Detection Plate, Capped
1400: ABgene 96 PCR Detection Plate Mark II, Capped
2100: ABgene SuperPlate Raised Semi-Skirt 96-Well PCR Plate, Easy Peal Heat Seal
2400: ABgene SuperPlate Semi-Skirted 96-Well PCR Plate, Capped
2800: ABgene SuperPlate Skirted 96-Well PCR Plate
84: ABgene 384 PCR Plate, Easy Peel Heat Seal
937: ABgene 384 PCR Plate Mark II, Easy Peel Heat Seal
1111: ABgene 384 Diamond PCR Plate, Diamond Foil Seal
Step 4
Conc. & final
reaction volume		 2: 1.1X concentration, 25μl final reaction volume
4: 1.1X concentration, 50μl final reaction volume
6: 2X concentration, 25μl final reaction volume
8: 2X concentration, 50μl final reaction volume
Step 5
Final MgCl2
concentration
(NOT for EX)		 15: 1.5mM MgCl2
20: 2.0mM MgCl2
25: 2.5mM MgCl2
30: 3.0mM MgCl2
35: 3.5mM MgCl2
40: 4.0mM MgCl2
Step 6
ReddyMix (NOT for TS)		 LD: ReddyMix (or leave blank for standard)
Step 7
Pack size		 A: 96 tubes of type 337, 620, 489 or 350 (25μl reaction volume)
96 tubes of type 337, 620, 489 or 350 (50μl reaction volume)
12 strips of type 266, 1182, 772 or 773 (25μl reaction volume)
12 strips of type 266, 1182, 772 or 773 (50μl reaction volume)
8 strips of type 1113, 1114, 848 or 849 (25μl reaction volume)
8 strips of type 1113, 1114, 848 or 849 (50μl reaction volume)
B: 10 x 96 tubes of type 337, 620, 489 or 350 (25μl reaction volume)
10 x 96 tubes of type 337, 620, 489 or 350 (50μl reaction volume)
10 x 12 strips of type 266, 1182, 772 or 773 (25μl reaction volume)
10 x 12 strips of type 266, 1182, 772 or 773 (50μl reaction volume)
10 x 8 strips of type 1113, 1114, 848 or 849 (25μl reaction volume)
10 x 8 strips of type 1113, 1114, 848 or 849 (50μl reaction volume)
C: 10 x 96-well plate of type 600, 700, 800, 900, 990, 1000, 1100, 1400, 1900, 2100, 2400, 2800 (25μl)
5 x 96-well plate of type 600, 700, 800, 900, 990, 1000, 1100, 1400, 1900, 2100, 2400, 2800 (50μl)
10 x 384-well plate of type 384, 937, 1111 (25μl)