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Pierce Catalog

Detoxi-Gel Endotoxin Removal Gel

Eliminate worries about endotoxins interfering with your test results.

Detoxi-Gel Endotoxin Removing Gel and Columns provide a quick method for removal of endotoxins (lipopolysaccharides or LPS) from samples. Even low levels of endotoxins can be toxic to cells or organisms and must be removed before biological samples can be introduced.

Highlights:

  • Resin uses immobilized polymixin B to remove pyrogens by binding their lipid A domains
  • Reduces endotoxin levels in protein solutions, cell culture media, solutions containing pharmacological components and aqueous buffers
  • 1 ml of gel removes >9,995 EU from a 10,000 EU challenge of LPS (greater than 99% efficiency)
  • 1 ml of gel binds 4000-6000 E.U. of the lipopolysaccharide from E. Coli strain 055:BS
  • Protein recovery dependent upon protein type and concentration; some empirical testing required
  • Stable and reusable; resin can be regenerated by stripping off the endotoxins with a 1% deoxycholate solution in pyrogen-poor water; no loss in binding capacity even after 10 regenerations

References:

  1. Kohda, C., et al. (2002). Infect. Immun. 70(3), 1334-1341.
  2. Wang, L., et al. (2002). J. Clin. Invest. 110(8), 1175-1184.
  3. Lei, B., et al. (2002). Infect. Immun. 70(12), 6880-6890.
  4. Kimoto, T., et al. (2003). Infect. Immun. 71(5), 2447-2454.
  5. Madrazo, D.R., et al. (2003). Infect. Immun. 71(9), 5418-5421.
  6. Goh, Y.Y., (2002). Appl. Environ. Microbiol. 68(12), 6343-6352.
  7. Lavelle, E.C., et al. (2003). J. Immunol. 171, 2384-2392.
  8. Ben-Menachem, G., et al. (2003). Proc. Natl. Acad. Sci. USA 100(13), 7913-7918.
Cat.# 製品名 容 量 価格
20339 Detoxi-Gel Endotoxin Removing Gel 10 ml ¥27,000
20340 Detoxi-Gel Endotoxin Removing Gel 1,000 ml ¥683,000
20344 Detoxi-Gel Endotoxin Removing Columns 5 x 1 ml ¥27,000