Precise Protein Gels
製品問合せ MSDS
 
Pierce Catalog

Precise Protein Gels

Precise Protein Gels are cast in a durable plastic cassette using a neutral pH buffer that prevents polyacrylamide breakdown and results in a long shelf life. High-resolution staining and transfer of proteins is accomplished quickly on these 1 mm thick gels. Gels are individually packaged in an easy-to-open plastic pouch and are ready to run with no comb or tape to remove. Following electrophoresis, the cassettes open easily with no special tools required and little chance of damaging the gel. Precise Gels are available in gradient (4-20% and 8-16%) and fixed (8%, 10% and 12%) concentrations and in 10-, 12- and 15-well formats.

Highlights:

  • 12-month guarantee ensures consistent performance
  • 45-minute run time provides results quickly
  • Unique running buffer produces excellent separation and high-resolution protein bands
  • Sample wells hold up to twice the volume of Novex gels (10-well=50 µl, 12-well=30 µl, 15-well=25 µl)
  • Stains quickly and with high sensitivity using coomassie and silver stains
  • Transfers efficiently to nitrocellulose and PVDF membranes in just 90 minutes
  • Compatible with Laemmli sample buffer
  • Compatible with standard mini-gel tanks so there is no need to purchase new equipment

Precise Protein Gels utilize a unique Tris-HEPES-SDS running buffer to improve band resolution and reduce run-time. The buffer can be made according to the recipe provided in the instruction booklet or purchased premixed.

Migration Table

Compatible Gel Tanks
BioRad® Mini-PROTEAN™ II and 3 Hoefer Mini VE System (SE 300)
Novex® Xcell I, II™ and Surelock™ Thermo Scientific Owl P8 Systems
IBI Universal Protein System C.B.S. Scientific MGV 302/402
EC 4-Cell Daiichi Mini 2-Gel & 6-Gel
Hoefer Tall Mighty Small™ (SE 280) GradiGel Mini 4-Cell
Hoefer Mighty Small™ II (SE 260/ SE 250) Fisher Biotech ™ Vertical Minigel System

Gel Specifications

  • Cassette size: 10 cm x 8.5 cm x 4.5 mm
  • Gel size: 8 cm x 5.8 cm x 1 mm
  • Shelf life: 12 months @ 4°C
  • Running buffer: Tris-HEPES-SDS
  • Sample buffer: Tris-HCl-SDS
Cat.# Description 容 量 価格 MSDS
25200 8% Precise Protein Gels, 10 wells x 50µl ea. 10 gels ¥25,000 BID-P165
25201 10% Precise Protein Gels, 10 wells x 50 µl ea. 10 gels ¥25,000 BID-P165
25202 12% Precise Protein Gels, 10 wells x 50 µl ea. 10 gels ¥25,000 BID-P165
25203 8-16% Precise Protein Gels, 10 wells x 50 µl ea. 10 gels ¥25,000 BID-P165
25204 4-20% Precise Protein Gels, 10 wells x 50 µl ea. 10 gels ¥25,000 BID-P165
25220 8% Precise Protein Gels, 12 wells x 30 µl ea. 10 gels ¥25,000 BID-P165
25221 10% Precise Protein Gels, 12 wells x 30 µl ea. 10 gels ¥25,000 BID-P165
25222 12% Precise Protein Gels, 12 wells x 30 µl ea. 10 gels ¥25,000 BID-P165
25223 8-16% Precise Protein Gels, 12 wells x 30 µl ea. 10 gels ¥25,000 BID-P165
25224 4-20% Precise Protein Gels, 12 wells x 30 µl ea. 10 gels ¥25,000 BID-P165
25240 8% Precise Protein Gels, 15 wells x 25 µl ea. 10 gels ¥25,000 BID-P165
25241 10% Precise Protein Gels, 15 wells x 25 µl ea. 10 gels ¥25,000 BID-P165
25242 12% Precise Protein Gels, 15 wells x 25 µl ea. 10 gels ¥25,000 BID-P165
25243 8-16% Precise Protein Gels, 15 wells x 25 µl ea. 10 gels ¥25,000 BID-P165
25244 4-20% Precise Protein Gels, 15 wells x 25 µl ea. 10 gels ¥25,000 BID-P165
28380 BupH Tris-Glycine Transfer Buffer
Each pack yields 500 ml of 25 mM Tris, 192 mM glycine, pH 8 when dissolved in 400 ml distilled water and 100 ml methanol (20 liters total).
40 pack ¥25,000  
28398 BupH Tris-HEPES-SDS Running Buffer
Each pack yields 500 ml of 100 mM Tris, 100 mM HEPES, 3 mM SDS, pH 8 ± 0.5 when dissolved in 500 ml distilled water (5 liters total).
10 packs ¥8,200 BID-P286