Krypton Infrared Protein Stain - Fluorescent
Compatible with LI-COR Odyssey and other infrared imaging systems
Krypton* Infrared Protein Stain† is a fluorescent stain for detecting proteins in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and 2-D gels. Researchers now have an easy-to-use, high-performance fluorescent protein stain for the near-infrared region of the spectrum compatible with the LI-COR Odyssey* Infrared Imaging System and other commonly available CCD instruments (Figure 1). The stain delivers substantial improvements in protein-staining performance compared to coomassie stains (Figure 2). Krypton Infrared Protein Stain exhibits minimal protein-to-protein variation and provides high signal intensity with a linear response to staining (Figure 3-4).
Highlights:
- Excitation/emission maxima – 690/718 nm
- Instrument-compatible – ideal for LI-COR Odyssey Instruments and other CCD instrumentation
- Versatile – compatible with mass spectrometry
- Wide quantitative range – three to four orders of magnitude
- Sensitive – 0.25 ng protein can be detected with the basic protocol (~2 hours)
- Fast – detect down to 2 ng protein with the rapid protocol (~1 hour)
Figure 1. Krypton Infrared Protein Stain protocol
Figure 2. Krypton Infrared Protein Stain is up to 130 times more sensitive than coomassie stain.
Proteins were separated in 4-20% Tris-glycine gels and stained with Krypton Infrared Protein Stain or coomassie stain (GelCode* Blue Stain Reagent, Product # 24592). The gels were imaged with the Odyssey Infrared Imaging System at 680nm excitation and 720 nm emission.
Figure 3. Krypton Infrared Protein Stain produces a linear response to staining with minimal protein-to-protein variation.
Relative fluorescent intensity was plotted as a function of protein quantity for proteins of various sizes and containing post-translational modifications. The relative fluorescent intensity for each data point is the average value for triplicate gels.
Figure 4. Krypton Infrared Protein Stain provides exceptional sensitivity and low background in 2-D analysis.
Processed HeLa cell protein extract (28 µg) was focused on a pH 5-8 IPG strip followed by 4-20% SDS-PAGE. The gel was stained using the basic protocol (~2 hours) and imaged with the Odyssey Infrared Imaging System using the 700 nm channel.
| Cat.# | 製品名 | 容 量 | 価格 | MSDS |
| 53070 | Krypton Infrared Protein Stain (10X) Sufficient reagent to stain 4 mini gels (8 x 10 cm) |
20 ml | ¥9,500 | BID-P052 |
| 53071 | Krypton Infrared Protein Stain (10X) Sufficient reagent to stain 20 mini gels (8 x 10 cm) or two to four large-format gels |
100 ml | ¥27,000 | BID-P052 |
| 53072 | Krypton Infrared Protein Stain (10X) Sufficient reagent to stain 100 mini gels (8 x 10 cm) or 10 to 20 large-format gels. |
500 ml | ¥98,000 | BID-P052 |
