Beta'-COP Polyclonal Antibody
Coatomer proteins are involved in regulating transport between the endoplasmic reticulum (ER) and the Golgi complex and in intra-Golgi transport. There exist two coatomer-protein mechanisms (COPI and COPII) and although they have mechanistic parallels, they are molecularly distinct. The COPI coat is comprised of seven subunits (alpha-, beta-, beta'-, gamma-, delta-, epsilon-, and zeta-COP) in a complex called coatomer. Assembly of the coatomer (COPI) onto non-clathrin coated vesicles is regulated by ADP-ribosylation factor (ARF). Vesicle formation, budding, fusion, and disassembly is dependent on GDP-GTP exchange, COPI, and ARF. COPI has been shown to facilitate retrograde intracellular transport from the ER to the Golgi complex. By contrast, COPII facilitates anterograde transport between these subcellular organelles. COPII has been shown to be independently and selectively recruited to the ER relative to COPI subunits.
Beta'-COP Polyclonal Antibody (product #89912) serves as an excellent marker for Golgi. It detects coatomer-protein I beta' (COPI beta) from rat cells.
Beta'-COP Polyclonal Antibody has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~115 kDa protein representing COP beta from PC-12 cell extract. A non-specific band at ~100 kDa may be seen.
Beta'-COP Polyclonal Antibody immunogen is a synthetic peptide corresponding to residues K(494) V L A A Q E T H E G V T E D G I E(511) of rat beta-COP.
Storage: -20C, Avoid Freeze/Thaw Cycles
Applications: Western Blotting, 2 µg/ml dilution
| Cat.# | 製品名 | 容 量 | 価格 |
| 89912 | Beta'-COP Polyclonal Antibody (Anti-coatomer-protein I beta’ or COPI beta’) Organelle target: Golgi 50 µg of epitope affinity purified rabbit IgG (1 mg/ml) in PBS containing 1 mg/ml BSA and 0.05% sodium azide. |
50 µg | 販売終了 |
