HDAC1 Polyclonal Antibody
Chromatin is a highly specialized structure composed of tightly compacted chromosomal DNA. Gene expression within the nucleus is controlled, in part, by a host of protein complexes which continuously pack and unpack the chromosomal DNA. One of the known mechanisms of this packing and unpacking process involves the acetylation and deacetylation of the histone proteins comprising the nucleosomal core. Acetylated histone proteins confer accessibility of the DNA template to the transcriptional machinery for expression. Histone deacetylases (HDACs) are chromatin remodeling factors that deacetylate histone proteins and thus, may act as transcriptional repressors. HDACs are classified by their sequence homology to the yeast HDACs and there are currently 2 classes. Class I proteins are related to Rpd3 and members of class II resemble Hda1p. HDAC1 is a class I histone deacetylase containing 482 amino acid residues. HDAC1 has been shown to interact directly with transcription factors and has been shown to deacetylate histone proteins H3 and H4.
HDAC1 Polyclonal Antibody (product #89910) detects histone deacetylase 1 (HDAC1) from human, mouse, rat, hamster and canine tissues and cells.
HDAC1 Polyclonal Antibody has been successfully used in Western blot, immunoprecipitation, ChIP, immunofluoresence, immunocytochemistry and immunohistochemistry procedures. By Western blot, this antibody detects an ~62 kDa protein representing HDAC1 from HeLa cell lysate. Immunocytochemical staining of HDAC1 in HeLa cells with HDAC1 Polyclonal Antibody results in nuclear staining.
The HDAC1 Polyclonal Antibody immunogen is a synthetic peptide corresponding to residues C E(467) E K P E A K G V K E E V K L A(482) of human HDAC1.
Storage: -20C, Avoid Freeze/Thaw Cycles
Applications: Western Blotting, 0.5 µg/ml dilution
Figure 1. Nuclei enrichment from tissue.
Liver and kidney tissue (400 mg each) were processed using the Nuclei Enrichment Kit for Tissue. Total cell lysate and isolated nuclei were analyzed by Western blotting for histone deacetylase (HDAC), a soluble nuclear marker.
| Cat.# | 製品名 | 容 量 | 価格 |
| 89910 | HDAC1 Polyclonal Antibody (Anti-histone deacetylase 1) Organelle target: Nuclei 50 µg of epitope affinity purified rabbit IgG (1 mg/ml) in PBS containing 1 mg/ml BSA and 0.05% sodium azide. |
50 µg | 販売終了 |
