VDAC Polyclonal Antibody
The Voltage-Dependent Anion Channel (VDAC or mitochondrial Porin) is an outer membrane mitochondrial protein. The VDAC protein is thought to form the major pores through which adenine nucleotides are transferred through the outer mitochondrial membrane.
VDAC has also been implicated in the formation of the mitochondrial permeability transition pore complex in apoptotic cells. This complex, formed by VDAC, adenine nucleotide translocator (ANT), and cyclophilin D (CypD), is thought to allow the mitochondria to undergo metabolic uncoupling and irreversible morphologic changes that ultimately destroy the mitochondria during apoptosis.
VDAC Polyclonal Antibody (product #89913) serves as an excellent marker for mitochondria. It detects voltage-dependent anion channel (VDAC) from rat tissues. VDAC Polyclonal Antibody (product #89913) has been successfully used in Western blot procedures. By Western blot, this antibody detects a single, prominent ~31 kDa band representing VDAC from rat heart extract.
VDAC Polyclonal Antibody (part #89913) immunizing peptide corresponds to the C-terminal amino acid residues 185-197 of human VDAC1. This sequence is completely conserved between isoforms 1, 2 and 3 of human VDAC.
Storage: -20C, Avoid Freeze/Thaw Cycles
Applications: Western Blotting, 2 µg/ml dilution
| COX4 | VDAC | Cytochrome C | ||||
| M | C | M | C | M | C | |
| 試薬法 |  |
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| ダウンス法 |  |
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M: mitochondria, C: Cytosol
Table 1 - Analysis of mitochondrial integrity.
Mitochondria and cytosolic fractions were prepared from fresh rat liver and heart tissue samples using the reagent-based and Dounce homogenization methods. Fractions were analyzed via Western Blot for VDAC, COX4 and Cytochrome C. SuperSignal West Pico Chemiluminescent Substrate (Product# 34080) was used for detection. VDAC (Voltage-dependent Anion Channel) is a membrane protein found on the outer membrane of the mitochondria, COX4 is an inner mitochondria membrane protein, and Cytochrome C resides in the intermembrane
| Cat.# | 製品名 | 容 量 | 価格 |
| 89913 | VDAC Polyclonal Antibody (Anti-voltage-dependent anion channel) Organelle target: Mitochondria 50 µg of epitope affinity purified rabbit IgG (1 mg/ml) in PBS containing 1 mg/ml BSA and 0.05% sodium azide. |
50 µg | 販売終了 |
