pierce 製品問合せ MSDS
 
Pierce Catalog

Human IFN gamma Antibodies

IFN gamma (IFNg) antibody matched pair for ELISA, ELISPOT, and Western blotting.

Thermo Scientific Pierce IFN gamma monoclonal and polyclonal antibodies enable measurement of human interferon gamma (IFNg) in ELISA, ELISPOT, and Western Blot. These human IFNg antibodies provide excellent high specificity and sensitivity. The M700A / M701B monoclonal antibody matched pair is very well-published and comes highly recommended for ELISA and ELISPOT applications. The detecting antibody can be purchased biotin-labeled or in a purified, carrier-free format.

IFN gamma, also called Type II interferon, macrophage activation factor, and immune interferon is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE it appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific and includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFNg is involved in cytokine regulation and also acts synergistically with other cytokines.

Cat # Type Format Isotype Clone# Cross-Reactivity> Applications
M700A Purified F, PF, CF Mouse IgG1/K 2G1 Hu ELISA (ref1,4,5,8,9,12,16)
Matched Pair: Coat
ELISPOT (ref6,10-13,15,17,18)
WB
M701 Purified F, PF, CF Mouse IgG1 B133.5 Hu ELISA (ref2,3,4,14,16)
ELISPOT (ref6)
WB
M701B Biotin-labeled F, P, C Mouse IgG1 B133.5 Hu ELISA (ref1,5,8,9,12)
Matched Pair: Detect
ELISPOT (ref10-13,15,17,18)
WB
P700 Purified F, PF, CF Rabbit Polyclonal Hu ELISA (ref7,16)
WB

Format Codes: F=frozen, L=lyophilized, C=carrier, CF=carrier-free, P=preservative, PF=preservative-free

See info-sheets (PDF) in the ordering information table below for additional product-specific information.

Related IFN gamma Products

参考文献

  1. Battistini, L., et al. (1997). Phenotypic and cytokine analysis of human peripheral blood delta gamma T cells expressing NK cell receptors. J. Immunol. 159, 3723-3730.
  2. Blotta, M.H., et al. (1997) Corticosteroids inhibit IL-12 production in human monocytes and enhance their capacity to induce IL-4 synthesis in CD4+ lymphocytes. J. Immunol. 158, 5589-5595.
  3. Blotta, M.H., et al. (1996). Cross-linking of the CD40 ligand on human CD4+ T lymphocytes generates a costimulatory signal that up-regulates IL-4 synthesis. J. Immunol. 156, 3133-3140.
  4. Fujimoto, T., et al. (1997). Streptococcal preparation OK-432 is a potent inducer of IL-12 and a T helper cell 1 dominant state. J. Immunol. 158, 5619-5626.
  5. Hess, S.D., et al. (2003). Human CD4+ T cells present within the microenvironment of human lung tumors are mobilized by the local and sustained release of IL-12 to kill tumors in situ by indirect effects of IFNγ. J. Immunol. 170, 400-412.
  6. Hesse, M.D., et al. (2001). A T Cell Clone’s Avidity is a Function of Its Activation State. J. Immunol. 167, 1353-1361.
  7. Hoft, D.F., et al. (1998). Bacile Calmette-Guerin vaccination enhances human T cell responsiveness to mycobacteria suggestive of a memoryu-like phenotype. J. Immunol. 161, 1045-1054.
  8. Kallmann, B.A., et al. (1997). Systemic bias of cytokine production toward cell-mediated immune regulation in IDDM and toward humoral immunity in Graves' disease. Diabetes. 46, 237-243.
  9. Koelle, D.M., et al. (2001). CD8 CTL from genital herpes simplex lesions: recognition of viral tegument and immediate early proteins and lysis of infected cutaneous cells. J. Immunol. 166: 4049-4058.
  10. Leong, S., et al. (1998). Digital quantification of enzyme-linked immunospot (ELISPOT). Biotechniques. 25(5), 830-836.
  11. Lyke, K.E. et al. (2005). HLA-A2 supertype-restricted cell-mediated immunity by peripheral blood mononuclear cells derived from Malian children with severe or uncomplicated plasmodium falciparum malaria and healthy controls. Infect. Immun. 73(9), 5799-5808.
  12. Malhotra, I., et al. (1997). In utero exposure to helminth and mycobacterial antigens generates cytokine responses similar to that observed in adults. J. Clin. Invest. 99, 1759-1766.
  13. Manfras, B.J., et al. (2004). Impeded Th1 CD4 memory T cell generation in chronic-persisting liver infection with Echinococcus multilocularis. Int. Immunol. 16(1), 43-50.
  14. Marshall, J.D., et al. (1995). IL-12 inhibits the production of IL-4 and IL-10 in allergen-specific human CD4+ T lymphocytes. J. Immunol. 155, 111-117.
  15. Salerno-Gonçalves, R. et al. (2004). Immunization of volunteers with salmonella enterica serovar typhi strain Ty21a elicits the oligoclonal expansion of CD8+ T cells with predominant Vβ repertoires. Infect. Immun. 73(6), 3521-3520.
  16. Targan, S.R., et al. (1995). Definition of a lamina propria T cell responsive state. Enhanced cytokine responsivemess of T cells stimulated through the CD2 pathway. J. Immunol. 154, 664-675.
製品情報
Cat # 製　品　名 容 量 価 格 MSDS
M700A Human IFN gamma MAb (clone 2G1)
 500 µg ¥63,000  
M701 Human IFN gamma MAb (clone B133.5) 500 µg ¥64,000  
M701B Human IFN gamma Biotinylated MAb (clone B133.5) 250 µg ¥62,000 BID-P270
P700 Human IFN gamma PAb
 1 mg ¥53,000