mouse ifng antibody#1
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Pierce Catalog

Mouse IFNg (Interferon gamma) Antibodies

IFN gamma (IFNg) antibodies for ELISA, ELISPOT, and Neutralization.

Thermo Scientific Pierce IFN gamma monoclonal and polyclonal antibodies enable measurement of mouse interferon gamma (IFNg) in immunoassays. Both clones are frequently cited in publications for neutralization and have been used together successfully for ELISA. We offer two different clones but recommend a self-sandwich (clone XMG1.2 = MM700 + MM700B) for quantification of mouse IFN gamma in ELISA. Because IFNg is a homodimer, the same binding site is available for both capture and detection, and the monoclonal self-sandwich assay is possible. Product MM701, clone R4-6A2, has also been used successfully in ELISPOT.

Our purified antibodies are supplied in a low-endotoxin, preservative-free, carrier-free format that is ideal for custom conjugations or plate coating for ELISA or ELISPOT.

Cat # Type Format Isotype Clone# Cross-Reactivity Applications
MM700 Purified L, PF, CF Rat IgG1 XMG1.2 Mo ELISA (ref4,18,25,26,28) Matched Pair: Coat In vitro Neutralization (ref4,7,9,11,14,22,23) In vivo Neut. (ref10,13,17) IH-frozen (ref3,15) WB
MM700B Biotin-labeled L, P, C Rat IgG1 XMG1.2 Mo ELISA (ref1,19) Matched Pair: Detect Intracellular flow (ref12)
MM701 Purified L, PF, CF Rat IgG1 R4-6A2 Mo ELISA (ref1,5,18,19,24-26,28) In vitro Neut. (ref18) In vivo Neut. (ref5,8,20) IH-frozen (ref27) Intracellular flow (ref2) WB

The following list of poly- and monoclonals have been used to select against specific human interferons.

製品情報
Cat # 製　品　名 Pkg.Size 価 格
221001 Mouse IFN alpha Mab
(clone RMMA-1)
 250 µg ¥66,000
321001 Mouse IFN alpha PAb
 2x104 ¥59,000
324001 Mouse IFN beta PAb
 2x104 ¥59,000

Format Codes: F=frozen, L=lyophilized, C=carrier, CF=carrier-free, P=preservative, PF=preservative-free

See info-sheets (PDF) in the ordering information table below for additional product-specific information.

Related IFN gamma Products

参考文献

  1. Akbari, O., et al. (2008). ICOS/ICOSL interaction is required for CD4+ invariant NKT cell function and homeostatic survival. J. Immunol. 180, 5448-5456.
  2. Assenmacher, M., et al. (1998). Commitment of individual Th1-like lymphocytes to expression of IFN-gamma versus IL-4 and IL-10: selective induction of IL-10 by sequential stimulation of naïve Th cells with IL-12 and IL-4. J. Immunol. 161, 2825-2832.
  3. Bogen, S.A., et al. (1993). Analysis of IL-2, IL-4, and IFNγ producing cells in situ during immune responses to protein antigens. J. Immunol. 150, 4197-4205.
  4. Bradley, L.M., et al. (1995). The cytokines IL-4, IFNg, and Il-12 regulate the development of subsets of memory effector T cells In Vitro. 155, 1713-1724.
  5. Chaudhri, G., et al. (2004). Polarized type 1 cytokine response and cell-mediated immunity determine genetic resistance to mousepox. PNAS, 101(24), 9057-9062.
  6. Chu, R.S., et al. (1997). CpG oligodeozynucleotides act as adjuvants that switch on T helper 1 (Th1) immunity. J. Exp. Med. 186(10), 1623-1631.
  7. Cohn, L., et al. (1998). IL-4 independent induction of airway hyperesponsivness by Th2, but not Th1, cells. J. Immunol. 161, 3813-3816.
  8. Flamand, V., et al. (1998). CD40 ligation prevents neinatal induction of transplantation tolerance. J. Immunol. 160, 4666-4669.
  9. Goňi, O., et al. (2002). Immunosuppression during acute Trypanosoma cruzi infection: involvement of Ly6G (Gr1+)CD11b+ immature myeloid suppressor cells. Int. Immunol. 14(10), 1125-1134.
  10. Gorczynski, R.M., et al. (1994). A role for non-specific (Cyclosporin A) or specific (monoclonal antibodies to ICAM-1, LFA, and IL-10) immunomodulation in the prolongation of skin allografts after antigen-specific pretransplant immunization or transfusion. J. Immunol. 152, 2011-2019.
  11. Grohmann, U., et al. (2001). Positive regulatory role of IL-12 in macrophages and modulation by IFN-γ. J. Immunol. 167, 221-227.
  12. Homey, B., et al. (1998). Topical FK506 suppresses cytokine and costimulatory molecule expression in epidermal and local draining lymph node cells during primary skin immune responses. J. Immunol. 160, 5331-5340.
  13. Kohler, J. et al. (1993). IFNγ involvement in the severity of gram-negative infections in mice. J. Immunol. 151, 916-921.
  14. Miner, K.T., et al. (1998). Generation, persistence, and nodulation of Th) effector cells: role of autocrine IL-4 and IFN-gamma. J. Immunol. 160, 5280-5287.
  15. Morris, S.C., et al. (1994). Effects of IL-12 on In Vivo cytokine gene expression and Ig Isotype selection. J. Immunol. 152, 1047-1056.
  16. Mottram, P.L., et al. (1998). Cardiac allografts from IL-4 knockout recipients: assessment of transplant arteriosclerosis and peripheral tolerance. J. Immunol. 161, 602-609.
  17. Parent, M.A., et al. (2006). Gamma interferon, tumor necrosis factor alpha, and nitric oxide synthase 2, key elements of cellular immunity, perform critical protective functions during humoral defense against lethal pulmonary Yersinia pestis infection. Infect. Immun. 74(6), 3381-3386
  18. Pearlman, E. et al. (1993). Modulation of murine cytokine responses to mycobacterial antigens by helminth-induced T helper 2 cell responses. J. Immunol. 151, 4857-4864.
  19. Rolph, M.S., et al. (2000). Partially TAP-independent protection against Listeria monocytes by H2-M3-Restricted CD8+ T cells. J. Immunol. 165, 4575-4580.
  20. Rosendahl, A., et al. (1998). Perforin and IFN-gamma are involved in the antutumor effect of antibody-targeted superantigens. J. Immunol. 160, 5309-5313.
  21. Taguchi, T., et al. (1990). Analysis of Th1 and Th2 cells in murine gut-associated tissues: frequencies of CD4+ and CD8+ T cells that secrete IFNg and IL-5. J. Immunol. 145, 68-77.
  22. Targoni, O.S., et al. (1998). Endogenous myelin basic protein inactivates the high avidity T cell repertoire. J. Exp. Med. 187, 2055-2063.
  23. Tobin, J.F., et al. (1993). Transfected Leishmania expressing biologically active IFNγ. J. Immunol. 150, 5059-5069.
  24. Tokoyoda, K., et al. (2004). Up-regulation of IL-4 production by the activated cAMP/cAMP-dependent protein kinase (protein kinase A) pathway in CD3/CD28-stimulated naïve T cells. Int. Immunol. 16(5), 643-653.
  25. Tomura, M., et al. (1998). Differential capacities of CD4+, CD8+, and CD4-CD8-T cell subsets to express IL-18 receptor and produce IFN gamma in response to IL-18. J. Immunol. 160, 3759-3765.
  26. Tomura, M., et al. (1998). A critical role for IL-18 in the proliferation and activation of NK1.1+ CD3-cells. J. Immunol. 160, 4738-4746.
  27. Tsung, K., et al. (1998). Immune response against large tumors eradicated by treatment with cyclophosphamide and IL-12. J. Immunol. 160, 1369-1377.
  28. Yeung, V.P., et al. (1998). Heat-killed listeria monoctogenes as an adjuvant converts established murine Th2-dominated immune response into Th1-dominated responses. J. Immunol. 161, 4146-4152.
製品情報
Cat # 製　品　名 容 量 価 格 MSDS
MM700 Mouse IFN gamma MAb (clone XMG1.2)
 500µg ¥56,000  
MM700B Mouse IFN gamma Biotinylated MAb (clone XMG1.2)
 250µg ¥63,000 BID-P270
MM701 Mouse IFN gamma MAb (clone R4-6A2) 500µg ¥56,000