About PHF-Tau and Other Neuroscience Antibodies
What is Tau protein? What is PHF-tau?
Paired helical filament (PHF) is a major component of the neurofibrillary tangles involved in the pathology of Alzheimer’s disease. PHFs are composed of the microtubule-associated protein tau in a hyper-phosphorylated state (ref1). Tau protein is produced by a single gene expressed predominantly in neurons. The Tau gene undergoes complex alternative splicing, yielding six different isoforms of tau in the adult brain. Following translation, the tau protein can be further modified by phosphorylation at several different sites (refs2,3).
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Anti-PHF-tau Monoclonal Antibodies
Identification of hyperphosphorylated tau regions and the development of assays for early Alzheimer's diagnosis relies on phosphorylation-dependent monoclonal antibodies that recognize disease-specific phosphorylation patterns. Anti-PHF-tau antibody clone AT8 has been shown to detect PHF-tau doubly phosphorylated at Ser202 and Thr205, serines 199 and 202, and serines 205 and 208 (ref4). Anti-PHF-tau antibody AT8 has been used extensively to bind PHF-tau in neurofibrillary tangles in samples from Alzheimer's patients (refs4-7). Anti-PHF-tau antibody clones AT100, AT180, and AT270 have also been used successfully to stain neurofibrillary tangles containing PHF-tau (ref7). Clone AT180 recognizes PHF-tau phosphorylated at Thr231. Clone AT270 recognizes PHF-tau phosphorylated at Thr181. Use of these antibodies has demonstrated a significant increase in phosphorylation at these sites in Alzheimer's disease (ref3). Clone AT100 recognizes PHF-tau phosphorylated at Ser212 and Thr214.
| Product | Clone # | Specificity | Format | Price |
| MN1020 | AT8 | PHF-tau (Ser202/Thr205)a | Purified | ¥85,000 |
| MN1020B | AT8 | PHF-tau (Ser202/Thr205)a | Biotin-labeled | ¥85,000 |
| MN1040 | AT180 | PHF-tau (Thr231) | Purified | ¥85,000 |
| MN1040B | AT180 | PHF-tau (Thr231) | Biotin-labeled | ¥85,000 |
| MN1050 | AT270 | PHF-tau (Thr181) | Purified | ¥88,000 |
| MN1060 | AT100 | PHF-tau (Ser212/Thr214) | Purified | ¥88,000 |
| MN1000 | HT7 | Tau | Purified | ¥43,000 |
| MN1000B | HT7 | Tau | Biotin-labeled | ¥43,000 |
| MN1010 | BT2 | Tau | Purified | ¥43,000 |
| MN1010B | BT2 | Tau | Biotin-labeled | ¥43,000 |
| PN1000 | Sheep polyclonal | Tau | Purified | ¥62,000 |
a AT8 was shown to be cross-reactive to doubly phosphorylated motifs containing either serine 199 and 202 or serine 205 and 208 (ref4).
| Product | Clone # | Specificity | Format | Price |
| MN1150 | 10H3 | Beta Amyloid | Purified | ¥65,000 |
| MN1150B | 10H3 | Beta Amyloid | Biotin-labeled | ¥65,000 |
| MN1160 | MID-N3, H14 | γ-γ Enolase | Purified | ¥58,000 |
| MN1080 | NM4 | GAP-43, B-50 | Purified | ¥65,000 |
| MN1090 | NM2 | GAP-43, B-50 | Purified | ¥65,000 |
| MN1180 | MIG-G2 | GFAP: Glial Fibrillary Acidic Protein | Purified | ¥65,000 |
| MN1190 | MIG-N18 | Neurofilament (Ser) | Purified | ¥28,000 |
| MN1250 | 2B10 | Rab3a | Purified | ¥55,000 |
| MN1280 | 11D2 | SNAP25 | Purified | ¥55,000 |
| MN1270 | 15C2 | Synapsin | Purified | ¥55,000 |
| MN1260 | 5C8 | Synapsin | Purified | ¥55,000 |
| MN1290 | 9B6 | Synapsin | Purified | ¥55,000 |
| MN1170 | MIG-T4, NFT200 | Tangles | Purified | ¥62,000 |
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参考文献
- Goedert, M. (1996). Tau protein and the neurofibrillary pathology of Alzheimer’s disease. Ann. N. Y. Acad. Sci. 777: 121-131.
- Kosik, K.S. (1990). Tau protein and neurodegeneration. Mol. Neurobiol. 4(3-4): 171-179.
- Goedert, M., et al. (1994). Epitope mapping of monoclonal antibodies to the paired helical filaments of Alzheimer’s disease: identification of phosphorylation sites in tau protein. Biochem. J. 301(Pt 3): 871-877.
- Porzig, R., et al. (2007). Epitope mapping of mAbs AT8 and Tau5 directed against hyperphosphorylated regions of the human tau protein. Biochem. Biophys. Res. Commun. 358(2): 644-649.
- Arima, K., et al. (2000). NACP/alpha-synuclein and tau constitute two distinctive subsets of filaments in the same neuronal inclusions in brains from a family of parkinsonism and dementia with Lewy bodies: double-immunolabeling fluorescence and electron microscope studies. Acta Neuropathol. 100(2): 115-121.
- Uchihara, T., et al. (2001). Evolution from pretangle neurons to neurofibrillary tangles monitored by thiazen red combined with Gallyas method and double immunofluorescence. Acta Neuropathol. 101(6): 535-539.
- Spillantini, M. G., et al. (1996). Comparison of the neurofibrillary pathology in Alzheimer’s disease and familial presenile dementia with tangles. Acta Neuropathol. 92(1): 42-48.
神経科学関連抗体
- About PHF-Tau and Other Neuroscience Antibodies
- Beta-Amyloid Antibodies
- Gamma gamma Enolase Antibodies
- Glial Fibrillary Acidic Protein Antibodies
- Growth Associated Protein B-50 (GAP43) Antibodies
- MAP-2 Antibodies
- Neurofilament Antibodies
- Neuron Specific Enolase Antibodies
- PHF-tau Antibodies
- Rab3a Antibodies
- SNAP25 Antibodies
- Synapsin Antibodies
- Synuclein Antibodies
- Tangles Antibodies
- Tau Antibodies
