Easy-Titer ELIFA System

Effective ELISA alternative allows immunoassays to be completed in just 25 minutes!

特長:

• Effective ELISA device allows immunoassays to be completed in just 25 minutes (each step is reduced to a 5 minute filtration)
• Ideal for ELISA-type immunoassays, dot blots and nucleic acid blots
• Binding kinetics of any ELISA step performed using Easy-Titer System overrides limiting diffusion
• Greater sensitivity because transfer membranes bind more protein than plastic surfaces
• 96-well arrangement is compatible with 96-well microplates and multichannel pipettes
• Most wash steps are eliminated, further reducing assay time
• Cross-talk between samples is eliminated due to the use of tight seals
• Results are quantitated easily by simply drawing color solution into a microplate and reading using a standard ELISA reader
• Use insoluble or precipitating substrates to create dot blot results

Figure 1. Easy-Titer ELIFA System diagram.
Colored product is filtered into microplate wells. Precipitated colored product is deposited on membrane.

Figure 2. Checkerboard titration results using a precipitating substrate.
A checkerboard titration is a single experiment in which the concentration of two components is varied in a way to visualize the best signal-to-noise ratio conditions.

参考文献:

1. Paffard, S.M., et al. (1996). J. Immunol. Methods 192, 133-136.
2. Liu, Z.-H., et al. (2001). Proc. Natl. Acad. Sci. USA 98, 7289-7294.
3. Menalled, L.B., et al. (2002). J. Neurosci. 22, 8266-8276.

Images

Product Image for Product Family Easy-Titer ELIFA System

ELIFAシステム

• Easy-Titer ELIFA System

洗浄バッファー、停止バッファー

• ELISA Wash Buffer
• Stop Solution