Microarray Slides for Covalent Protein Coating

An economical alternative to nitrocellulose coated slides

Aminopropylsilane (APS), Epoxysilane (EPS) and Aldehyde microarray slides are used for the covalent attachment of biological molecules, including proteins and DNA to enable the researcher to perform complex analysis of biological samples. Each product is a 1 in x 3 in glass slide in a conventional format and an enhanced surface (ES) format. The ES format has an extensively micro-etched surface that produces a smaller, more uniform spot size. This can help in producing higher quality arrays compared to the conventional, non-etched format. The screw cap slide container is a complimentary product to be use for incubation of slides with various reagents. Its purpose is to save on the volume of reagents used.

Aminoproplylsilane microarray slides are used for non-covalent adsorption of protein. Epoxysilane microarray slides are reactive with lysine, arginine, cysteine and hydroxyls at pH 5-9. Aldehyde microarray slides are reactive with lysine and arginine where pH 7-10 drives Schiff's base reaction.

Generally, an antibody or protein solution can be spotted or printed on many different types of media, including 1 x 3 inch glass microscope slides, glass plates, polystyrene microplates or membranes (e.g., nitrocellulose). An antibody microarray is the printing of antibodies in a very small spot on the medium, allowing construction of an array with many different antibody specificities in the small space of, in this case, a 1 x 3 inch glass microscope slide. Arraying antibodies with different specificities allows the testing of single or multiple samples for many targets. After antibodies are printed in a defined array, a conventional ELISA protocol is performed and the completed assay slide is evaluated on a slide reader adapted to you detection method. There are many fluorescent slide readers in laboratories due to the genomic era of DNA microarrays.

References

Key Reviews

1. Angenendt, P. (2003). Next generation of protein support materials: Evaluation for protein and antibody microarray applications. J. Chrom. A. 1009, 97-104.
2. Angenendt, P. (2002) Toward optimizated antibody microarrays: a comparison of current microarray support materials. Anal. Biochem. 309, 253-260.
3. Kusnezow, W. (2002). Antibody Microarrays: Promises and Problems. BioTechniques. 33:514, 14-23.
4. Kusnezow, W. (2003) Antibody microarrays: An evaluation of production parameters. Proteomics. 3, 254-264.
5. Angenendt, P. (2005) Progress in protein and antibody microarray technology. Drug Discov. Today. 10 (7), 503.

Aminopropylsilane

1. Mezzasoma, L. (2002). Antigen Microarrays for Serodiagnosis of Infectious Diseases. Clinical Chem. 48 (1), 121-130.
2. Fang, Y. (2002). G-protein-coupled receptor microarrays. Chembiochem. 3(10), 987-91.
3. Miller, J. (2003) Antibody microarray profiling of human prostate cancer sera: Antibody screening and identification of potential biomarkers. Proteomics 3, 56-63.

Epoxysilane

1. Angenendt, P. (2004) Seeing better through a MIST: evaluation of monoclonal recombinant antibody fragments on microarrays. Anal. Chem. 76 (10), 2916-21.
2. Olle, E., (2005) Comparison of antibody array substrates and the use of glycerol to normalize spot morphology. Exp & Molec Pathology. 79(3):206-9.
3. Sapsford, K.E. (2004) Real-time analysis of protein adsorption to a variety of thin films. Biosensors and Bioelectronics. 19, 1045-1055.

Aldehyde

1. Bradbury, A (2003). Antibodies in proteomics II: screening, high-throughput characterization and downstream applications. Trends Biotechn 21(7), p312.
2. Du, H. (2005). Parallel Detection and Quantification Using Nine Immunoassays in a Protein Microarray for Drug and Serum Samples. Biomedical Microdevices. 7 (2), 143
3. Espejo, A. (2004) Protein-domain microarrays. Methods Mol. Biol. 264, 173-81.
4. Haab, B, (2003) Methods and applications of antibody microarrays in cancer research. Proteomics. 3, 2116.
5. Miller, J. (2003). Antibody microarray profiling of human prostate cancer sera: Antibody screening and identification of potential biomarkers. Proteomics. 3, 56-63.
6. Poetz, O (2005) Protein microarray for antibody profiling: Specificity and affinity determination on a chip. Proteomics. 5, 2402.

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• Microarray Slides for Covalent Protein Coating