BSA and BGG Protein Assay Standard Sets
Selection of a protein standard is potentially the greatest source of error in any protein assay. The best choice for a standard is a purified, known concentration of the predominant protein found in the samples. This is not always possible or necessary; in some cases, all that is needed is a rough estimate of the total protein concentration in the sample. If a highly purified version of the protein of interest is not available or it is too expensive to use as the standard, the alternative is to choose a protein that will produce a very similar color response curve with the selected protein assay method. For general protein assay work, bovine serum albumin (BSA) works well for a protein standard because it is widely available in high purity and relatively inexpensive. Although it is a mixture containing several immunoglobulins, bovine gamma globulin (BGG) also is a good standard when determining the concentration of antibodies, since BGG produces a color response curve that is very similar to that of immunoglobulin G (IgG).
Typical protein assays are used to determine protein concentration by comparing sample assay response to that of a standard whose concentration is known. Protein samples and protein standards are processed in the same manner by mixing them with assay reagent and using a spectrophotometer to measure the absorbances. Pierce offers protein standards for life scientists interested in accurately quantitating the amount of protein in their samples.
Bovine Serum Albumin Standard
- The most widely-used protein assay standard
- Sterile-filtered, stable and consistent
- BSA is an ideal standard for BCA Assays
- Ampules ensure product integrity
- 2 mg/ml BSA solution
Bovine Gamma Globulin Standard
- Sterile-filtered, stable and consistent
- BGG is an ideal standard for Coomassie (Bradford) Assays
- Ideal standard for measuring antibody concentration with any protein assay
- Ampules ensure product integrity
- 2 mg/ml BGG solution
For greatest accuracy in estimating total protein concentration in unknown samples, it is essential to include a standard curve each time the assay is performed. This is particularly true for the protein assay methods that produce non-linear standard curves. Deciding on the number of standards and replicates used to define the standard curve depends upon the degree of non-linearity in the standard curve and the degree of accuracy required. In general, fewer points are needed to construct a standard curve if the color response is linear. Typically, standard curves are constructed using at least two replicates for each point on the curve.
Features
- Stable and sterile filtered
- Ideal for BCA and Bradford-based Protein Assays
- Pre-Diluted Standard Curve Range: 125-2,000 µg/ml for BCA, Coomassie and Bradford Assays
- Sufficient materials to prepare 15-35 standard tube protocol curves or 175-350 standard microplate protocol curves running duplicate data points
Benefits
- Construct a standard curve series as fast as you can pipette
- Convenient — With the Pre-diluted BSA and BGG there is no need to prepare a diluted standard series for each determination
- More reliable protein quantitation because of the assured accuracy of the concentrations of each standard
- Dramatically improved speed to result, especially with Bradford-based protein assays
| Cat.# | 製品名 | 容 量 |
| 23208 | Pre-Diluted Protein Assay Standards: Bovine Serum Albumin (BSA) Set Diluted in 0.9% saline and preserved with 0.05% NaN3 Includes: 7 x 3.5 ml of standardized BSA solutions each at a specific concentration along a range from 125-2,000 µg/ml |
Set |
| 23209 | 2 mg/ml Bovine Serum Albumin Standard Ampules | 10 x 1 ml |
| 23210 | 2 mg/ml Bovine Serum Albumin Standard | 50 ml |
| 23212 | Bovine Gamma Globulin Standard Ampules, 2mg/ml | 10 x 1 ml |
| 23213 | Pre-Diluted Protein Assay Standards: Bovine Gamma Globulin (BGG) Set Diluted in 0.9% saline and preserved with 0.05% NaN3 Includes: 7 x 3.5 ml of standardized BGG solutions each at a specific concentration along a range from 125-2,000 µg/ml |
Set |
