蛍光色素標識
ビオチン標識(ビオチン化)法は、高感度検出が必要なELISAやウェスタンブロッティングなどのアッセイシステムにおいて、検出可能なプローブを作成するために広く利用されています。
Emission |
Fluor |
Ex/Em† |
e†† |
Spectrally Similar Dyes |
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Blue |
DyLight 405 |
400/420 |
30K |
Alexa 405, Cascade Blue |
|
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Green |
DyLight 488 |
493/518 |
70K |
Alexa 488, fluorescein, FITC |
|
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Yellow |
DyLight 549 |
550/568 |
150K |
Alexa 546, Alexa 555, Cy3,TRITC |
|
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Red |
DyLight 594 |
593/618 |
80K |
Alexa 594, Texas Red |
|
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Red |
DyLight 633 |
638/658 |
170K |
Alexa 633 |
|
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Red |
DyLight 649 |
646/674 |
250K |
Alexa 647, Cy5 |
|
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Near IR |
DyLight 680 |
682/715 |
140K |
Alexa 680, Cy5.5 |
|
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Near IR |
DyLight 750 |
752/778 |
210K |
Alexa 750 |
|
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Infrared |
DyLight 800 |
770/794 |
270K |
IRDye* 800 |
蛍光色素標識キット
蛍光色素標識試薬
| Emission | Fluor | Available Derivatives | |||
| NHS Ester | Maleimide | Phosphine | |||
| Blue | DyLight 350 |
Yes | Yes | ||
| Blue | DyLight 405 |
Yes | Yes | ||
| Green | DyLight 488 |
Yes | Yes | Yes | |
| Yellow | DyLight 550 |
Yes | Yes | Yes | |
| Red | DyLight 594 |
Yes | Yes | ||
| Red | DyLight 633 |
Yes | Yes | ||
| Red | DyLight 650 |
Yes | Yes | Yes | |
| Near IR | DyLight 680 |
Yes | Yes | ||
| Near IR | DyLight 747 |
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| Near IR | DyLight 755 |
Yes | Yes | ||
| Infrared | DyLight 800 |
Yes | Yes | ||













